GAN Ming, DING De-Cheng, ZHOU Yuan-Cong1*
( Institute of Plant Physiology and Ecology, Shanghai Institutes for Biomedical Sciences, the Chinese Academy of Sciences, Shanghai 200032, China)
Abstract By using of DE52 Cellulose, Sephadex
G-150 and FPLC Q Sepharose chromatography, a storage protein (SP) was purified
from Aphis craccivora Koch and its subunit was characterized. Its
molecular weight was about 60 kD as determined by SDS-PAGE analysis under both
reducing and non-reducing conditions, and its pI was about 5.0. It was a
glycoprotein. The protein accumulated in larval hemolymph, and decreased when
host turned to adult, and could be detected in adults in very low
concentration. According to the molecular weight, amino acids composition, and
its dynamic alteration of concentrations, the protein should be a persisting
storage protein of hemimetabolous insects.
Key words Aphis
craccivora Koch; storage protein; purification
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